Stable expression of the chlorocatechol dioxygenase gene from Ralstonia eutropha NH9 in hybrid poplar cells.
Identifieur interne : 003480 ( Main/Exploration ); précédent : 003479; suivant : 003481Stable expression of the chlorocatechol dioxygenase gene from Ralstonia eutropha NH9 in hybrid poplar cells.
Auteurs : Yasunori Ohmiya [Japon] ; Toshiro Ono ; Toru Taniguchi ; Naoei Itahana ; Naoto Ogawa ; Kiyotaka Miyashita ; Kunio Ohmiya ; Kazuo Sakka ; Tetsuya KimuraSource :
- Bioscience, biotechnology, and biochemistry [ 1347-6947 ] ; 2009.
Descripteurs français
- KwdFr :
- Chromatographie en phase liquide (MeSH), Cupriavidus necator (enzymologie), Dioxygenases (génétique), Gènes de plante (MeSH), Populus (cytologie), Populus (génétique), Réaction de polymérisation en chaîne (MeSH), Technique de Southern (MeSH), Technique de Western (MeSH), Végétaux génétiquement modifiés (MeSH).
- MESH :
- cytologie : Populus.
- enzymologie : Cupriavidus necator.
- génétique : Dioxygenases, Populus.
- Chromatographie en phase liquide, Gènes de plante, Réaction de polymérisation en chaîne, Technique de Southern, Technique de Western, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Dioxygenases.
- cytology : Populus.
- enzymology : Cupriavidus necator.
- genetics : Populus.
- Blotting, Southern, Blotting, Western, Chromatography, Liquid, Genes, Plant, Plants, Genetically Modified, Polymerase Chain Reaction.
Abstract
The cbnA gene encoding chlorocatechol dioxygenase from the soil bacterium Ralstonia eutropha NH9 under the control of a modified cauliflower mosaic virus 35S promoter was introduced into a hybrid poplar (Populus tremula x P. tremuloides). Integration of the cbnA gene in transgenic poplar was confirmed by PCR and genomic Southern blot analysis. Expression of the cbnA gene was analyzed by Western blot analysis. Transgenic poplar calli efficiently converted 3-chlorocatechol to 2-chloro-cis,cis-muconate.
DOI: 10.1271/bbb.80848
PubMed: 19502754
Affiliations:
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last="Itahana">Naoei Itahana</name></author><author><name sortKey="Ogawa, Naoto" sort="Ogawa, Naoto" uniqKey="Ogawa N" first="Naoto" last="Ogawa">Naoto Ogawa</name></author><author><name sortKey="Miyashita, Kiyotaka" sort="Miyashita, Kiyotaka" uniqKey="Miyashita K" first="Kiyotaka" last="Miyashita">Kiyotaka Miyashita</name></author><author><name sortKey="Ohmiya, Kunio" sort="Ohmiya, Kunio" uniqKey="Ohmiya K" first="Kunio" last="Ohmiya">Kunio Ohmiya</name></author><author><name sortKey="Sakka, Kazuo" sort="Sakka, Kazuo" uniqKey="Sakka K" first="Kazuo" last="Sakka">Kazuo Sakka</name></author><author><name sortKey="Kimura, Tetsuya" sort="Kimura, Tetsuya" uniqKey="Kimura T" first="Tetsuya" last="Kimura">Tetsuya Kimura</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19502754</idno><idno type="pmid">19502754</idno><idno type="doi">10.1271/bbb.80848</idno><idno 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sort="Ono, Toshiro" uniqKey="Ono T" first="Toshiro" last="Ono">Toshiro Ono</name></author><author><name sortKey="Taniguchi, Toru" sort="Taniguchi, Toru" uniqKey="Taniguchi T" first="Toru" last="Taniguchi">Toru Taniguchi</name></author><author><name sortKey="Itahana, Naoei" sort="Itahana, Naoei" uniqKey="Itahana N" first="Naoei" last="Itahana">Naoei Itahana</name></author><author><name sortKey="Ogawa, Naoto" sort="Ogawa, Naoto" uniqKey="Ogawa N" first="Naoto" last="Ogawa">Naoto Ogawa</name></author><author><name sortKey="Miyashita, Kiyotaka" sort="Miyashita, Kiyotaka" uniqKey="Miyashita K" first="Kiyotaka" last="Miyashita">Kiyotaka Miyashita</name></author><author><name sortKey="Ohmiya, Kunio" sort="Ohmiya, Kunio" uniqKey="Ohmiya K" first="Kunio" last="Ohmiya">Kunio Ohmiya</name></author><author><name sortKey="Sakka, Kazuo" sort="Sakka, Kazuo" uniqKey="Sakka K" first="Kazuo" last="Sakka">Kazuo Sakka</name></author><author><name sortKey="Kimura, Tetsuya" sort="Kimura, Tetsuya" uniqKey="Kimura T" first="Tetsuya" last="Kimura">Tetsuya Kimura</name></author></analytic><series><title level="j">Bioscience, biotechnology, and biochemistry</title><idno type="eISSN">1347-6947</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Blotting, Southern (MeSH)</term><term>Blotting, Western (MeSH)</term><term>Chromatography, Liquid (MeSH)</term><term>Cupriavidus necator (enzymology)</term><term>Dioxygenases (genetics)</term><term>Genes, Plant (MeSH)</term><term>Plants, Genetically Modified (MeSH)</term><term>Polymerase Chain Reaction (MeSH)</term><term>Populus (cytology)</term><term>Populus (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Chromatographie en phase liquide (MeSH)</term><term>Cupriavidus necator (enzymologie)</term><term>Dioxygenases (génétique)</term><term>Gènes de plante (MeSH)</term><term>Populus (cytologie)</term><term>Populus (génétique)</term><term>Réaction de polymérisation en chaîne (MeSH)</term><term>Technique de Southern (MeSH)</term><term>Technique de Western (MeSH)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Dioxygenases</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Cupriavidus necator</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Cupriavidus necator</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Dioxygenases</term><term>Populus</term></keywords><keywords scheme="MESH" 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Integration of the cbnA gene in transgenic poplar was confirmed by PCR and genomic Southern blot analysis. Expression of the cbnA gene was analyzed by Western blot analysis. Transgenic poplar calli efficiently converted 3-chlorocatechol to 2-chloro-cis,cis-muconate.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19502754</PMID><DateCompleted><Year>2009</Year><Month>10</Month><Day>06</Day></DateCompleted><DateRevised><Year>2009</Year><Month>06</Month><Day>24</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1347-6947</ISSN><JournalIssue CitedMedium="Internet"><Volume>73</Volume><Issue>6</Issue><PubDate><Year>2009</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Bioscience, biotechnology, and biochemistry</Title><ISOAbbreviation>Biosci Biotechnol Biochem</ISOAbbreviation></Journal><ArticleTitle>Stable expression of the chlorocatechol dioxygenase gene from Ralstonia eutropha NH9 in hybrid poplar cells.</ArticleTitle><Pagination><MedlinePgn>1425-8</MedlinePgn></Pagination><Abstract><AbstractText>The cbnA gene encoding chlorocatechol dioxygenase from the soil bacterium Ralstonia eutropha NH9 under the control of a modified cauliflower mosaic virus 35S promoter was introduced into a hybrid poplar (Populus tremula x P. tremuloides). Integration of the cbnA gene in transgenic poplar was confirmed by PCR and genomic Southern blot analysis. Expression of the cbnA gene was analyzed by Western blot analysis. Transgenic poplar calli efficiently converted 3-chlorocatechol to 2-chloro-cis,cis-muconate.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ohmiya</LastName><ForeName>Yasunori</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Forest Tree Breeding Center, Hitachi, Ibaraki, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ono</LastName><ForeName>Toshiro</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Taniguchi</LastName><ForeName>Toru</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Itahana</LastName><ForeName>Naoei</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Ogawa</LastName><ForeName>Naoto</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Miyashita</LastName><ForeName>Kiyotaka</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Ohmiya</LastName><ForeName>Kunio</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Sakka</LastName><ForeName>Kazuo</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Kimura</LastName><ForeName>Tetsuya</ForeName><Initials>T</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>06</Month><Day>07</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Biosci Biotechnol Biochem</MedlineTA><NlmUniqueID>9205717</NlmUniqueID><ISSNLinking>0916-8451</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>EC 1.13.11.-</RegistryNumber><NameOfSubstance UI="D049308">Dioxygenases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 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UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>6</Month><Day>9</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>6</Month><Day>9</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>10</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19502754</ArticleId><ArticleId IdType="pii">JST.JSTAGE/bbb/80848</ArticleId><ArticleId IdType="doi">10.1271/bbb.80848</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country></list><tree><noCountry><name sortKey="Itahana, Naoei" sort="Itahana, Naoei" uniqKey="Itahana N" first="Naoei" last="Itahana">Naoei Itahana</name><name sortKey="Kimura, Tetsuya" sort="Kimura, Tetsuya" uniqKey="Kimura T" first="Tetsuya" last="Kimura">Tetsuya Kimura</name><name sortKey="Miyashita, Kiyotaka" sort="Miyashita, Kiyotaka" uniqKey="Miyashita K" first="Kiyotaka" last="Miyashita">Kiyotaka Miyashita</name><name sortKey="Ogawa, Naoto" sort="Ogawa, Naoto" uniqKey="Ogawa N" first="Naoto" last="Ogawa">Naoto Ogawa</name><name sortKey="Ohmiya, Kunio" sort="Ohmiya, Kunio" uniqKey="Ohmiya K" first="Kunio" last="Ohmiya">Kunio Ohmiya</name><name sortKey="Ono, Toshiro" sort="Ono, Toshiro" uniqKey="Ono T" first="Toshiro" last="Ono">Toshiro Ono</name><name sortKey="Sakka, Kazuo" sort="Sakka, Kazuo" uniqKey="Sakka K" first="Kazuo" last="Sakka">Kazuo Sakka</name><name sortKey="Taniguchi, Toru" sort="Taniguchi, Toru" uniqKey="Taniguchi T" first="Toru" last="Taniguchi">Toru Taniguchi</name></noCountry><country name="Japon"><noRegion><name sortKey="Ohmiya, Yasunori" sort="Ohmiya, Yasunori" uniqKey="Ohmiya Y" first="Yasunori" last="Ohmiya">Yasunori Ohmiya</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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